Technology (UPN: 40)

Technology A bacteriophage lysates production technology
Organisation Type Company
Organisation Region Middle East
Overview A method for the commercial production of bacteriophages composition. The method decreases the production volume and increases the production yields.

The innovative phage production process enables the following competitive advantages over conventional phage production by liquid fermentation:

  • Various High titer phage lysates produced concurrently in a small facility
  • Various Bacteria and Phages grown concurrently in separate and well controlled process, yielding reproducible batches :
  1. Rare contaminations with little material and time loss.
  2. Several phages may be produced in the same facility with small risk of cross contamination
  • Final titer 10^12 to 10^12 PFU allows for high-titer phage concentrations
  • Initial daily capacity of 10,000 Liters 10^10-1 PFU/ML
  • Soluble debris diluted to undetectable traces
  • Complete purification is effected by gradient centrifugation of the primary material
  • Low production cost of $0.05 / Gallon due to small use of consumables, culture media and reagents
  • A highly controlled Process resulting in reproducible batches

Possible Applications:

Topical phage ointments, Gasto-intestinal phage capsules, Acne protection phage Ointment, Dental phage products, Phage Probiotic adjuvant, Food Safety Phages food additives and surface treatment agents, Aquaculture Phages, E.coli Phage agent for livestock, Campylobacter phage, Crop protection phage agents, Fermentation control phages

Competitive Advantage

Conventional liquid fermentation of phage lysates is limited: • Large fermentors are expensive and bulky • Liquid fermentation is prone to contamination from external microbes resulting in lost production batches and long down time for sanitation • Fermentors are dedicated to single strain bacteria/phage at a time and require long and complex cleaning and validation procedures • Final titer is limited to 10^7 to 10^8 PFU (Plaque Forming Unit) • Yield is limited by fermentors capacity Conventional micro-filtration affects yield and quality because : 1) Remaining soluble debris is allergen and prohibited by regulation 2) Loss of several logs in titer • High production cost of over $10 / Gallon • Large qualitative and quantitative variations in batches

Development Stage:

In user / Testing result available

Intellectual Property:

US Patent granted in 2009

Innovator A company that develops technologies for the production of bacteriophages-based anti-bacterials for a range of clinical and animal applications.